data analysis

Complementary metaproteomic approaches to assess the bacterioplankton response toward a phytoplankton spring bloom in the Southern North Sea

Annually recurring phytoplankton spring blooms are characteristic of temperate coastal shelf seas. During these blooms, environmental conditions, including nutrient availability, differ considerably from non-bloom conditions, affecting the entire …

Influence of nanoLC column and gradient length as well as MS/MS frequency and sample complexity on shotgun protein identification of marine bacteria

Protein identification by shotgun proteomics, i.e., nano-liquid chromatography (nanoLC) peptide separation online coupled to electrospray ionization (ESI) mass spectrometry (MS)/MS, is the most widely used gel-free approach in proteome research. …

Non-Redfield, nutrient synergy and flexible internal elemental stoichiometry in a marine bacterium

The stoichiometric constraints of algal growth are well understood, whereas there is less knowledge for heterotrophic bacterioplankton. Growth of the marine bacterium Phaeobacter inhibens DSM 17395, belonging to the globally distributed Roseobacter …

Competition-induced starvation drives large-scale population cycles in Antarctic krill

Antarctic krill (Euphausia superba)—one of the most abundant animal species on Earth—exhibits a five to six year population cycle, with oscillations in biomass exceeding one order of magnitude. Previous studies have postulated that the krill cycle is …

No saturation in the accumulation of alien species worldwide

Although research on human-mediated exchanges of species has substantially intensified during the last centuries, we know surprisingly little about temporal dynamics of alien species accumulations across regions and taxa. Using a novel database of …

High performance CCD camera system for digitalisation of 2D DIGE gels

An essential step in 2D DIGE-based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge-coupled device (CCD) camera-based systems combined …

Unifying ecological stoichiometry and metabolic theory to predict production and trophic transfer in a marine planktonic food web

Two ecological frameworks have been used to explain multitrophic interactions, but rarely in combination: (i) ecological stoichiometry (ES), explaining consumption rates in response to consumers' demand and prey's nutrient content; and (ii) metabolic …

Native plasmids restrict growth of Phaeobacter inhibens DSM 17395: energetic costs of plasmids assessed by quantitative physiological analyses

Plasmid carriage is associated with energetic costs, and thus only those plasmids providing fitness benefits are stably maintained in the host lineage. Marine bacteria of the Roseobacter clade harbor up to 11 extrachromosomal replicons, adding …

Analysis of membrane–protein complexes of the marine sulfate reducer Desulfobacula toluolica Tol2 by 1D blue native‐PAGE complexome profiling and 2D blue native‐/SDS‐PAGE

Sulfate-reducing bacteria (SRB) obtain energy from cytoplasmic reduction of sulfate to sulfide involving APS-reductase (AprAB) and dissimilatory sulfite reductase (DsrAB). These enzymes are predicted to obtain electrons from membrane redox complexes, …

Predicting the spread of marine species introduced by global shipping

Predicting the arrival of alien species remains a big challenge, which is assumed to be a consequence of the complexity of the invasion process. Here, we demonstrate that spreading of alien marine species can be predicted by a simple model using only global shipping intensities, environmental variables, and species occurrence data. We provide species lists of the next potentially invading species in a local habitat or species causing harmful algal blooms with their associated probability of invasion. This will help to improve mitigation strategies to reduce the further introduction of alien species. Although this study focuses on marine algae, the model approach can be easily adopted to other taxonomic groups and their respective drivers of invasion